4D0T
GalNAc-T2 crystal soaked with UDP-GalNAc, EA2 peptide and manganese
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 77 |
| Collection date | 2013-10-05 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 117.217, 122.372, 249.724 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 249.720 - 2.450 |
| R-factor | 0.21429 |
| Rwork | 0.214 |
| R-free | 0.24116 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ffv |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.714 |
| Data reduction software | XDS |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 80.000 | 2.580 |
| High resolution limit [Å] | 2.450 | 2.450 |
| Rmerge | 0.110 | 0.800 |
| Number of reflections | 131982 | |
| <I/σ(I)> | 13.3 | 2.3 |
| Completeness [%] | 99.8 | 99.9 |
| Redundancy | 5.7 | 5.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
