4CYM
Complex of human VARP-ANKRD1 with Rab32-GppCp
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-02-06 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 144.445, 144.445, 135.680 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 125.090 - 2.800 |
| R-factor | 0.19466 |
| Rwork | 0.192 |
| R-free | 0.24565 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 1YHN AND 4B93 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.539 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0069) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 72.220 | 2.910 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.240 | 1.500 |
| Number of reflections | 40077 | |
| <I/σ(I)> | 8.8 | 0.9 |
| Completeness [%] | 98.8 | 94.1 |
| Redundancy | 7.4 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
