4CRL
Crystal structure of human CDK8-Cyclin C in complex with cortistatin A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-07-24 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 70.490, 71.252, 171.247 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 85.620 - 2.400 |
| R-factor | 0.20095 |
| Rwork | 0.199 |
| R-free | 0.25633 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3rgf |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.413 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 85.620 | 2.650 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.070 | 0.450 |
| Number of reflections | 34548 | |
| Completeness [%] | 94.9 | 98.6 |
| Redundancy | 2.8 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 20% PEG3350, 0.2M LITHIUM CHLORIDE, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K |
