4CQN
Crystal structure of the E.coli LeuRS-tRNA complex with the non- cognate isoleucyl adenylate analogue
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-05-11 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 155.620, 67.580, 224.590 |
| Unit cell angles | 90.00, 105.00, 90.00 |
Refinement procedure
| Resolution | 46.490 - 2.500 |
| R-factor | 0.21759 |
| Rwork | 0.214 |
| R-free | 0.29020 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4aq7 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.502 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 2.570 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.170 | 0.680 |
| Number of reflections | 76242 | |
| <I/σ(I)> | 7 | 1.8 |
| Completeness [%] | 97.1 | 98.7 |
| Redundancy | 3.2 | 3.18 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.5 | 0.1 M BIS-TRIS (PH 5.5), 23-25 % PEG 3350, 0-200 MM AMMONIUM ACETATE |
