4CQN

Crystal structure of the E.coli LeuRS-tRNA complex with the non- cognate isoleucyl adenylate analogue

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C (A, D)	LEUCINE--TRNA LIGASE	polymer	880	99516.0	2	UniProt (P07813) Pfam (PF09334) Pfam (PF13603) Pfam (PF00133) Pfam (PF08264)	ESCHERICHIA COLI K-12	LEUCYL-TRNA SYNTHETASE, LEURS, LEUCY-TRNA SYNTHETASE
2	B, D (B, E)	ESCHERICHIA COLI TRNA-LEU UAA ISOACCEPTOR	polymer	82	26475.7	2		ESCHERICHIA COLI
3	E, I (A, D)	ZINC ION	non-polymer		65.4	2	Chemie (ZN)
4	F, G, J, K (A, D)	N-[ISOLEUCINYL]-N'-[ADENOSYL]-DIAMINOSUFONE	non-polymer		458.5	4	Chemie (ILA)
5	H, L (B, E)	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
6	M, N, O (A, B, D)	water	water		18.0	306	Chemie (HOH)

Sequence modifications

A, D: 1 - 860 (UniProt: P07813)

PDB	External Database	Details
Met -19	-	expression tag
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	251983.5
Non-Polymers*	Number of molecules	8
	Total formula weight	2013.4
All*	Total formula weight	253996.9

*Water molecules are not included.