4CIM

Complex of a Bcl-w BH3 mutant with a BH3 domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Spacegroup name	P 1 21 1
Unit cell lengths	41.922, 60.726, 67.098
Unit cell angles	90.00, 105.85, 90.00

Refinement procedure

Resolution	44.229 - 1.500
R-factor	0.1874
Rwork	0.186
R-free	0.20960
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2y6w
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER (FOR MR)
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.590
High resolution limit [Å]	1.500	1.500
Rmerge	0.070	0.850
Number of reflections	51625
<I/σ(I)>	9.93	1.57
Completeness [%]	99.4	96.5
Redundancy	3.78	3.64

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		8		1 M TRISODIUM CITRATE 0.1 M IMIDAZOLE, PH 8.0