4CC9

Crystal structure of human SAMHD1 (amino acid residues 582-626) bound to Vpx isolated from sooty mangabey and human DCAF1 (amino acid residues 1058-1396)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-02-28
Detector	DECTRIS PILATUS 6M
Spacegroup name	P 21 21 21
Unit cell lengths	74.250, 82.881, 115.561
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.227 - 2.473
R-factor	0.1778
Rwork	0.176
R-free	0.21640
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	1.168
Data reduction software	XDS
Phasing software	autoSHARP
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.610
High resolution limit [Å]	2.460	2.560
Rmerge	0.090	0.530
Number of reflections	25520
<I/σ(I)>	15.9	3.6
Completeness [%]	99.3	97.8
Redundancy	6.5	6.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1				0.2 M MAGNESIUM CHLORIDE, 0.1 M HEPES PH 7.5, 15% PEG 400