4C7J
11b-Hydroxysteroid Dehydrogenase Type I in complex with inhibitor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E+ |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2011-05-17 |
| Detector | RAXIS HTC |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 95.686, 98.435, 99.112 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 68.610 - 2.160 |
| R-factor | 0.17718 |
| Rwork | 0.174 |
| R-free | 0.23956 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.646 |
| Data reduction software | MOSFLM |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.040 | 2.220 |
| High resolution limit [Å] | 2.160 | 2.160 |
| Rmerge | 0.120 | 0.550 |
| Number of reflections | 50932 | |
| <I/σ(I)> | 9.7 | 2.4 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 3.9 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
