4C1Q
Crystal structure of the PRDM9 SET domain in complex with H3K4me2 and AdoHcy.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-07-02 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 55.730, 78.180, 107.610 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.540 - 2.300 |
| R-factor | 0.21031 |
| Rwork | 0.208 |
| R-free | 0.25223 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ray |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.325 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.066 | 0.644 |
| Number of reflections | 21499 | |
| <I/σ(I)> | 13.7 | 2.3 |
| Completeness [%] | 99.5 | 99.3 |
| Redundancy | 4.3 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 5.5 | 278 | 0.2 M AMMONIUM SULFATE, 0.1 M BIS-TRIS PH 5.5 AND 25% W/V PEG3350 |
