4BZC
Crystal structure of the tetrameric dGTP-bound wild type SAMHD1 catalytic core
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 79.002, 138.727, 93.715 |
Unit cell angles | 90.00, 113.56, 90.00 |
Refinement procedure
Resolution | 49.710 - 2.880 |
R-factor | 0.19307 |
Rwork | 0.190 |
R-free | 0.24480 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3u1n |
RMSD bond length | 0.012 |
RMSD bond angle | 1.673 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.930 |
High resolution limit [Å] | 2.880 | 2.880 |
Rmerge | 0.120 | 0.900 |
Number of reflections | 40369 | |
<I/σ(I)> | 8.2 | 1.2 |
Completeness [%] | 95.6 | 97.4 |
Redundancy | 2.9 | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6.7 | 100 MM BIS-TRIS PH 6.7 AND 25% (W/V) PEG1500 |