4BSV
Heterodimeric Fc Antibody Azymetric Variant 1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CLSI BEAMLINE 08B1-1 |
Synchrotron site | CLSI |
Beamline | 08B1-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-09-12 |
Detector | MARRESEARCH MX-300HE |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 49.540, 74.920, 148.920 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 41.380 - 1.750 |
R-factor | 0.17926 |
Rwork | 0.178 |
R-free | 0.20821 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2j6e |
RMSD bond length | 0.008 |
RMSD bond angle | 1.317 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.000 | 1.840 |
High resolution limit [Å] | 1.750 | 1.750 |
Rmerge | 0.040 | 0.410 |
Number of reflections | 56316 | |
<I/σ(I)> | 26 | 3.9 |
Completeness [%] | 100.0 | 100 |
Redundancy | 7.3 | 7.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | VIA HANGING DROP VAPOR DIFFUSION METHOD AT A RATIO OF 2:1 OF 8.5 MG/ML OF AZYMETRIC VARIANT ABOVE A MOTHER LIQUOR SOLUTION COMPOSED OF 5% (V/V) ETHYLENE GLYCOL, 18% (W/V) POLYETHYLENE GLYCOL 3350, AND 0.15 M AMMONIUM IODIDE WITH AID OF MICROSEEDING. |