4BOF
Crystal structure of arginine deiminase from group A streptococcus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-12-14 |
| Detector | ADSC QUANTUM 210r |
| Spacegroup name | P 1 |
| Unit cell lengths | 87.910, 92.740, 120.970 |
| Unit cell angles | 96.15, 90.27, 100.13 |
Refinement procedure
| Resolution | 36.310 - 2.480 |
| R-factor | 0.2128 |
| Rwork | 0.211 |
| R-free | 0.24410 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1s9r |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.120 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.850 | 2.570 |
| High resolution limit [Å] | 2.480 | 2.480 |
| Rmerge | 0.150 | 0.980 |
| Number of reflections | 131658 | |
| <I/σ(I)> | 10.25 | 1.21 |
| Completeness [%] | 99.3 | 94.1 |
| Redundancy | 7.4 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.5 | 0.1M BIS-TRIS PH 5.5, 20% PEG3350, 0.3M LISO4, 25MG/ML PROTEIN |
