4BLW

Crystal structure of Escherichia coli 23S rRNA (A2030-N6)- methyltransferase RlmJ in complex with S-adenosylhomocysteine (AdoHcy) and Adenosine monophosphate (AMP)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-11-20
Detector	DECTRIS PILATUS 6M
Spacegroup name	P 1 21 1
Unit cell lengths	46.590, 77.060, 84.370
Unit cell angles	90.00, 99.50, 90.00

Refinement procedure

Resolution	45.951 - 1.950
R-factor	0.1686
Rwork	0.167
R-free	0.19910
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4blu
RMSD bond length	0.010
RMSD bond angle	1.315
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.000	2.050
High resolution limit [Å]	1.950	1.950
Rmerge	0.060	0.400
Number of reflections	41777
<I/σ(I)>	16	3.3
Completeness [%]	97.2	96.8
Redundancy	3.7	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		8.5		0.2 M SODIUM SULFATE DECAHYDRATE, 0.1 M TRIS-HCL PH 8.5 AND 30% W/V PEG 4000