4BL5
Crystal structure of human GDP-L-fucose synthase with bound NADP and product GDP-L-fucose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-09-29 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 1 |
| Unit cell lengths | 90.407, 90.478, 147.518 |
| Unit cell angles | 72.54, 72.64, 60.14 |
Refinement procedure
| Resolution | 29.660 - 2.600 |
| R-factor | 0.22482 |
| Rwork | 0.222 |
| R-free | 0.28565 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4b8w |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.380 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.640 | 2.640 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.140 | 1.150 |
| Number of reflections | 113998 | |
| <I/σ(I)> | 61.2 | 3.3 |
| Completeness [%] | 97.4 | 78.1 |
| Redundancy | 2.1 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.1M BIS-TRIS-PROPANE PH 7.5, 0.15M SODIUM CHLORIDE, 25% PEG 3350 |
