4BEA
Crystal Structure of eIF4E in Complex with a Stapled Peptide Derivative
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | BRUKER X8 PROTEUM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-10-21 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 38.301, 91.677, 137.908 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.880 - 2.570 |
R-factor | 0.22232 |
Rwork | 0.220 |
R-free | 0.26575 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4aza |
RMSD bond length | 0.009 |
RMSD bond angle | 1.355 |
Data reduction software | PROTEUM2 |
Data scaling software | PROTEUM2 |
Phasing software | PHASER |
Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 68.950 | 2.630 |
High resolution limit [Å] | 2.570 | 2.570 |
Rmerge | 0.090 | 0.460 |
Number of reflections | 8036 | |
<I/σ(I)> | 11.08 | 2.33 |
Completeness [%] | 98.8 | 83.2 |
Redundancy | 10.15 | 2.64 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 5.5 | 18-26% OF POLYETHYLENE GLYCOL 3350, 0.01-02M AMMONIUM SULPHATE AND 100MM BIS-TRIS AT PH 5.5 |