4BB2
Crystal structure of cleaved corticosteroid-binding globulin in complex with progesterone
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-02-12 |
| Detector | MARRESEARCH |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 89.190, 122.700, 81.930 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.070 - 2.480 |
| R-factor | 0.18293 |
| Rwork | 0.177 |
| R-free | 0.23393 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2vdy |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.276 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.000 | 2.630 |
| High resolution limit [Å] | 2.480 | 2.480 |
| Rmerge | 0.120 | 0.600 |
| Number of reflections | 16184 | |
| <I/σ(I)> | 12.9 | 3.2 |
| Completeness [%] | 99.6 | 97.6 |
| Redundancy | 7.2 | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 0.1 M HEPES 7.5, 2 % PEG 400, 2 M AMMONIUM SULFATE |
