4BAY

Phosphomimetic mutant of LSD1-8a splicing variant in complex with CoREST

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-1
Synchrotron site	ESRF
Beamline	ID14-1
Temperature [K]	100
Detector technology	CCD
Collection date	2011-12-12
Detector	ADSC QUANTUM 210
Spacegroup name	I 2 2 2
Unit cell lengths	117.980, 179.120, 233.640
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	59.060 - 3.100
R-factor	0.20901
Rwork	0.209
R-free	0.22583
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2x0l
RMSD bond length	0.005
RMSD bond angle	0.907
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.7.0027)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	65.000	3.270
High resolution limit [Å]	3.100	3.100
Rmerge	0.100	0.600
Number of reflections	44180
<I/σ(I)>	11.8	2.6
Completeness [%]	99.7	99.7
Redundancy	4.5	4.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	20DEG BY HANGING-DROP VAPOR DIFFUSION METHOD BY MIXING EQUAL VOLUMES OF PROTEIN SAMPLES WITH RESERVOIR SOLUTIONS CONTAINING 1.2 M SODIUM/POTASSIUM TARTRATE AND 100 MM N-(2-ACETAMIDO)IMINODIACETIC ACID, PH 6.5