4AYD
Structure of a complex between CCPs 6 and 7 of Human Complement Factor H and Neisseria meningitidis FHbp Variant 1 R106A mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-06-29 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 185.260, 54.010, 129.560 |
| Unit cell angles | 90.00, 118.03, 90.00 |
Refinement procedure
| Resolution | 15.000 - 2.400 |
| R-factor | 0.2038 |
| Rwork | 0.202 |
| R-free | 0.23060 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2w81 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.990 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 114.400 | 2.530 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.060 | 0.320 |
| Number of reflections | 43520 | |
| <I/σ(I)> | 11.8 | 3 |
| Completeness [%] | 97.3 | 86.1 |
| Redundancy | 3.1 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 9 | 20% PEG 6000, 0.1M BICINE PH 9.0 |
