4AY2

Capturing 5' tri-phosphorylated RNA duplex by RIG-I

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	287
Detector technology	CCD
Detector	ADSC QUANTUM 315
Spacegroup name	P 21 21 21
Unit cell lengths	47.745, 76.156, 221.222
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.900 - 2.800
R-factor	0.22153
Rwork	0.218
R-free	0.28583
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2ykg
RMSD bond length	0.007
RMSD bond angle	1.135
Refinement software	REFMAC (5.7.0029)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.700	2.950
High resolution limit [Å]	2.800	2.800
Rmerge	0.140	0.830
Number of reflections	20333
<I/σ(I)>	11.4	3
Completeness [%]	98.5	98.6
Redundancy	3.8	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1