4AVX
Hepatocyte Growth Factor-Regulated Tyrosine Kinase Substrate (Hgs-Hrs) bound to an IP2 compound at 1.68 A Resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-11-02 |
Detector | ADSC CCD |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 27.825, 67.545, 57.423 |
Unit cell angles | 90.00, 103.51, 90.00 |
Refinement procedure
Resolution | 55.830 - 1.680 |
R-factor | 0.18357 |
Rwork | 0.181 |
R-free | 0.23103 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3zyq |
RMSD bond length | 0.016 |
RMSD bond angle | 1.703 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 43.030 | 1.710 |
High resolution limit [Å] | 1.680 | 1.680 |
Rmerge | 0.040 | 0.180 |
Number of reflections | 22910 | |
<I/σ(I)> | 11.6 | 3.7 |
Completeness [%] | 97.0 | 93.3 |
Redundancy | 2.3 | 2.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 0.1 M BIS-TRIS PH 5.5; 25% PEG 3350 |