4AGU
CRYSTAL STRUCTURE OF THE HUMAN CDKL1 KINASE DOMAIN
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-08-05 |
| Detector | MARRESEARCH |
| Spacegroup name | P 32 |
| Unit cell lengths | 123.980, 123.980, 49.310 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.780 - 2.400 |
| R-factor | 0.1885 |
| Rwork | 0.186 |
| R-free | 0.22610 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4aaa |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.030 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.780 | 2.530 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.110 | 0.690 |
| Number of reflections | 33165 | |
| <I/σ(I)> | 8.1 | 2 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 4.8 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 17.5% PEG 3350, 0.1 M (NH4)CL PH 6.3, 0.05 M MG FORMATE |
