4AD7
Crystal structure of full-length N-glycosylated human glypican-1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | MAX II BEAMLINE I911-2 |
Synchrotron site | MAX II |
Beamline | I911-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-07-01 |
Detector | MARRESEARCH |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 47.250, 169.200, 151.710 |
Unit cell angles | 90.00, 94.96, 90.00 |
Refinement procedure
Resolution | 29.757 - 2.945 |
R-factor | 0.2211 |
Rwork | 0.218 |
R-free | 0.27810 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4acr |
RMSD bond length | 0.003 |
RMSD bond angle | 0.772 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX ((PHENIX.REFINE: DEV_938)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 3.030 |
High resolution limit [Å] | 2.950 | 2.950 |
Rmerge | 0.140 | 0.830 |
Number of reflections | 49679 | |
<I/σ(I)> | 15.4 | 3.4 |
Completeness [%] | 99.4 | 94.1 |
Redundancy | 8.9 | 8.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8 | 12-14% PEG 6000, 0.1 M TRIS-HCL, 0.2 M CACL2, PH 8.0 |