4A63
Crystal structure of the p73-ASPP2 complex at 2.6A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-07-04 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | I 1 2 1 |
| Unit cell lengths | 132.810, 170.100, 177.555 |
| Unit cell angles | 90.00, 91.98, 90.00 |
Refinement procedure
| Resolution | 85.050 - 2.270 |
| R-factor | 0.2166 |
| Rwork | 0.215 |
| R-free | 0.24410 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 2XWC AND 1YCS CHAIN B |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.080 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 88.720 | 2.790 |
| High resolution limit [Å] | 2.600 | 2.650 |
| Rmerge | 0.120 | 0.690 |
| Number of reflections | 111026 | |
| <I/σ(I)> | 8.4 | 2.1 |
| Completeness [%] | 97.4 | 95.6 |
| Redundancy | 3.7 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 1.00M NA/KPO4, 0.1M ACETATE PH 4.5 |
