3ZU7
Crystal structure of a designed selected Ankyrin Repeat protein in complex with the MAP kinase ERK2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | BRUKER AXS MICROSTAR |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2010-01-12 |
Detector | MAR scanner 345 mm plate |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 68.040, 89.370, 99.540 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.430 - 1.970 |
R-factor | 0.22113 |
Rwork | 0.219 |
R-free | 0.26928 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB ENTRIES 1ERK 1mj0 |
RMSD bond length | 0.023 |
RMSD bond angle | 1.957 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 5.410 | 1.880 |
High resolution limit [Å] | 1.870 | 1.870 |
Rmerge | 0.060 | 0.160 |
Number of reflections | 42412 | |
<I/σ(I)> | 19.21 | 8.3 |
Completeness [%] | 82.9 | 44.8 |
Redundancy | 5.2 | 4.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8.5 | 100 MM TRIS-HOAC (PH 8.5), 20% PEG4K, 5 MM CDCL2 |