3ZNI
Structure of phosphoTyr363-Cbl-b - UbcH5B-Ub - ZAP-70 peptide complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-10-15 |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 94.970, 131.800, 122.000 |
Unit cell angles | 90.00, 91.92, 90.00 |
Refinement procedure
Resolution | 30.540 - 2.210 |
R-factor | 0.1767 |
Rwork | 0.175 |
R-free | 0.21140 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB ENTRIES 3PFV 2ldr 4A49 AND 4AUQ |
RMSD bond length | 0.004 |
RMSD bond angle | 0.811 |
Phasing software | PHASER |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.500 | 2.270 |
High resolution limit [Å] | 2.210 | 2.210 |
Rmerge | 0.060 | 0.690 |
Number of reflections | 149230 | |
<I/σ(I)> | 14.2 | 2.1 |
Completeness [%] | 99.6 | 99.9 |
Redundancy | 3.4 | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 0.1 M BICINE, PH 9.0, 8-11% (W/V) PEG 3350 AND 0.1 M SODIUM FORMATE | |||
1 | 0.1 M BICINE, PH 9.0, 8-11% (W/V) PEG 3350 AND 0.1 M SODIUM FORMATE |