3ZM3
Catalytic domain of human SHP2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-02-26 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 |
| Unit cell lengths | 40.190, 42.091, 49.386 |
| Unit cell angles | 71.99, 81.09, 75.36 |
Refinement procedure
| Resolution | 19.530 - 1.500 |
| R-factor | 0.19249 |
| Rwork | 0.191 |
| R-free | 0.22747 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3b7o |
| RMSD bond length | 0.024 |
| RMSD bond angle | 2.012 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.590 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.040 | 0.040 |
| Number of reflections | 39749 | |
| <I/σ(I)> | 13.8 | 3.4 |
| Completeness [%] | 83.0 | 50.7 |
| Redundancy | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 16-22% (W/V) PEG3350, 0.1-0.2 M SODIUM CITRATE |
