3ZKG
BACE2 MUTANT APO STRUCTURE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-05-20 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 46.887, 88.466, 98.572 |
| Unit cell angles | 90.00, 96.58, 90.00 |
Refinement procedure
| Resolution | 32.820 - 1.900 |
| R-factor | 0.23061 |
| Rwork | 0.228 |
| R-free | 0.27272 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ewy |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.381 |
| Data reduction software | XDS |
| Data scaling software | SADABS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.800 | 1.990 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.010 | 0.350 |
| Number of reflections | 61792 | |
| <I/σ(I)> | 5.1 | 2.5 |
| Completeness [%] | 98.1 | 99.7 |
| Redundancy | 2.57 | 2.61 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 0.1M TRIS PH8, 25% PEG3350, pH 8.0 |
