3ZHR
Crystal structure of the H747A mutant of the SucA domain of Mycobacterium smegmatis KGD showing the active site lid closed
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-03-18 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 |
| Unit cell lengths | 79.970, 83.450, 159.670 |
| Unit cell angles | 99.64, 98.82, 100.98 |
Refinement procedure
| Resolution | 30.750 - 2.100 |
| R-factor | 0.1812 |
| Rwork | 0.180 |
| R-free | 0.20200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2yic |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.990 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | BUSTER (2.11.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.750 | 2.210 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.100 | 0.740 |
| Number of reflections | 221700 | |
| <I/σ(I)> | 10.7 | 1.9 |
| Completeness [%] | 96.6 | 92.1 |
| Redundancy | 3.9 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.6 | 52% MPD, 20 MM NA ACETATE, pH 7.6 |
