3WX2

Mouse Cereblon thalidomide binding domain, native

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	CCD
Collection date	2011-01-26
Detector	RAYONIX MX-225
Wavelength(s)	1.0000
Spacegroup name	I 21 3
Unit cell lengths	117.726, 117.726, 117.726
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	31.460 - 2.000
R-factor	0.19656
Rwork	0.195
R-free	0.22460
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3wx1
RMSD bond length	0.006
RMSD bond angle	1.011
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0049)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.000
Number of reflections	18492
Completeness [%]	100.0

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	5.7	277	0.1M sodium acetate (pH 5.7), 0.2M sodium sulphate, 12% PEG3350, 30% glycerol, VAPOR DIFFUSION, temperature 277K