3WWQ
Crystal structure of FAAP20 UBZ domain in complex with Lys63-linked diubiquitin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-10-24 |
Detector | RAYONIX MX225HE |
Wavelength(s) | 1.00000 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 59.767, 45.850, 172.900 |
Unit cell angles | 90.00, 98.06, 90.00 |
Refinement procedure
Resolution | 50.000 - 1.900 |
R-factor | 0.20957 |
Rwork | 0.208 |
R-free | 0.23458 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ubq |
RMSD bond length | 0.014 |
RMSD bond angle | 1.860 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0069) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.930 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.064 | 0.322 |
Number of reflections | 74073 | |
<I/σ(I)> | 27 | 4.26 |
Completeness [%] | 95.1 | 88.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 1.8M DL-malic acid, 0.1M glycine, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |