3WA0

Crystal structure of merlin complexed with DCAF1/VprBP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Collection date	2011-06-21
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	85.363, 135.760, 238.495
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.070 - 2.310
R-factor	0.2368
Rwork	0.235
R-free	0.26380
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1isn
RMSD bond length	0.007
RMSD bond angle	0.998
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.7.0032)

Data quality characteristics

	Overall
Low resolution limit [Å]	20.070
High resolution limit [Å]	2.300
Number of reflections	120906
Completeness [%]	99.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.8	277	0.2M ammonium fluoride, 23% PEG 3350, pH 6.8, VAPOR DIFFUSION, SITTING DROP, temperature 277K