3W9F
Crystal structure of the ankyrin repeat domain of chicken TRPV4 in complex with IP3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 90 |
| Detector technology | CCD |
| Collection date | 2012-06-08 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.00000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 105.094, 48.343, 132.145 |
| Unit cell angles | 90.00, 101.44, 90.00 |
Refinement procedure
| Resolution | 32.807 - 1.900 |
| R-factor | 0.2185 |
| Rwork | 0.218 |
| R-free | 0.24610 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3jxi |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.251 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.086 | 0.619 |
| Number of reflections | 100702 | |
| <I/σ(I)> | 18.2 | 2.37 |
| Completeness [%] | 97.2 | 93.3 |
| Redundancy | 5.1 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 110-120 mM sodium MES-HCl buffer pH 6.5, 1-2% PEG 4000, 11-12% MPD, 55-60 mM KH2PO4, vapor diffusion, sitting drop, temperature 293K |
