3VZ9
Crystal structure of the chicken Spc24-Spc25 globular domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-11-28 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 47.321, 58.137, 58.479 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.229 - 1.030 |
| R-factor | 0.1932 |
| Rwork | 0.193 |
| R-free | 0.20660 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ve7 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.092 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine: 1.7.2_869)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.050 |
| High resolution limit [Å] | 1.030 | 1.030 |
| Number of reflections | 79657 | |
| <I/σ(I)> | 49 | 5 |
| Completeness [%] | 99.2 | 99.2 |
| Redundancy | 5 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 0.12M Monosaccharides, 0.1M MES-Imidazol, 30% PEG20K/P550MME, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
