3VZ9

Crystal structure of the chicken Spc24-Spc25 globular domain

Summary for 3VZ9

• Entry DOI: 10.2210/pdb3vz9/pdb
• Related: 3VZA
• Descriptor: Uncharacterized protein, Spc24 protein (3 entities in total)
• Functional Keywords: rwd domain, kinetochore component, chromosome segregation, ndc80 complex, cell cycle
• Biological source: Gallus gallus (chicken); More
• Total number of polymer chains: 2
• Total formula weight: 20994.58

Authors

Nishino, T.,Fukagawa, T. (deposition date: 2012-10-09, release date: 2013-04-03, Last modification date: 2023-11-08)

Primary citation

Nishino, T.,Rago, F.,Hori, T.,Tomii, K.,Cheeseman, I.M.,Fukagawa, T.
CENP-T provides a structural platform for outer kinetochore assembly
Embo J., 32:424-436, 2013

PubMed Abstract: The kinetochore forms a dynamic interface with microtubules from the mitotic spindle during mitosis. The Ndc80 complex acts as the key microtubule-binding complex at kinetochores. However, it is unclear how the Ndc80 complex associates with the inner kinetochore proteins that assemble upon centromeric chromatin. Here, based on a high-resolution structural analysis, we demonstrate that the N-terminal region of vertebrate CENP-T interacts with the 'RWD' domain in the Spc24/25 portion of the Ndc80 complex. Phosphorylation of CENP-T strengthens a cryptic hydrophobic interaction between CENP-T and Spc25 resulting in a phospho-regulated interaction that occurs without direct recognition of the phosphorylated residue. The Ndc80 complex interacts with both CENP-T and the Mis12 complex, but we find that these interactions are mutually exclusive, supporting a model in which two distinct pathways target the Ndc80 complex to kinetochores. Our results provide a model for how the multiple protein complexes at kinetochores associate in a phospho-regulated manner.

PubMed: 23334297
DOI: 10.1038/emboj.2012.348

Experimental method

X-RAY DIFFRACTION (1.03 Å)