3VOW

Crystal Structure of the Human APOBEC3C having HIV-1 Vif-binding Interface

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	100
Detector technology	CCD
Collection date	2012-02-10
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9800
Spacegroup name	P 61
Unit cell lengths	105.042, 105.042, 70.046
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	90.970 - 2.150
R-factor	0.2162
Rwork	0.214
R-free	0.26260
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3ir2
RMSD bond length	0.011
RMSD bond angle	1.390
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	105.050	105.050	2.190
High resolution limit [Å]	2.150	5.840	2.150
Rmerge	0.054	0.037	0.338
Number of reflections	24043
<I/σ(I)>	20.1
Completeness [%]	99.9	98.9	100
Redundancy	22.3	20.9	22.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9	293	Bicine, PEG 6000, L-Arginine HCl, pH 9.0, vapor diffusion, hanging drop, temperature 293K