3VA2

Crystal structure of human Interleukin-5 in complex with its alpha receptor

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	CCD
Collection date	2009-11-21
Detector	ADSC QUANTUM 315
Wavelength(s)	1.000
Spacegroup name	P 21 21 21
Unit cell lengths	58.633, 88.680, 126.674
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.027 - 2.703
R-factor	0.2253
Rwork	0.223
R-free	0.27550
Structure solution method	SAD
RMSD bond length	0.003
RMSD bond angle	0.730
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	PHENIX ((phenix.refine: 1.7.3_928))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.900
High resolution limit [Å]	2.703	6.030	2.800
Rmerge		14.303	0.349
Number of reflections	18667
<I/σ(I)>	22.3	59.1	7.2
Completeness [%]	99.4	94.9	100
Redundancy	6.6	5.8	6.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.3	293	0.1M Na HEPES, 22% (v/v) PEG 4000, 2% i-Propanol, 6% (w/v) 1,6-Hexanediol , pH 7.3, VAPOR DIFFUSION, HANGING DROP, temperature 293K