3V6C
Crystal Structure of USP2 in complex with mutated ubiquitin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E+ SUPERBRIGHT |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-11-05 |
| Detector | RIGAKU SATURN A200 |
| Wavelength(s) | 1.54178 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 57.735, 84.077, 86.915 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 84.080 - 1.700 |
| R-factor | 0.17844 |
| Rwork | 0.176 |
| R-free | 0.22018 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2hd5 |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.983 |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 84.080 | 1.750 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.058 | |
| Number of reflections | 45718 | |
| <I/σ(I)> | 19.49 | 2.31 |
| Completeness [%] | 97.4 | 72.3 |
| Redundancy | 6.39 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 21% PEG 3350,0.2 M CaCl, 0.1 Bis-Tris pH 7.0, glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
