3V6C

Crystal Structure of USP2 in complex with mutated ubiquitin

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Ubiquitin carboxyl-terminal hydrolase 2	polymer	367	42171.7	1	UniProt (O75604) Pfam (PF00443)	Homo sapiens (human)	41 kDa ubiquitin-specific protease, Deubiquitinating enzyme 2, Ubiquitin thiolesterase 2, Ubiquitin-specific-processing protease 2
2	B (B)	Ubiquitin	polymer	91	10329.7	1	UniProt (P0CG48) Pfam (PF00240)	Homo sapiens (human)
3	C, F (A, B)	CHLORIDE ION	non-polymer		35.5	2	Chemie (CL)
4	D (A)	GLYCEROL	non-polymer		92.1	1	Chemie (GOL)
5	E (A)	ZINC ION	non-polymer		65.4	1	Chemie (ZN)
6	G, H (A, B)	water	water		18.0	290	Chemie (HOH)

Sequence modifications

A: 258 - 605 (UniProt: O75604)

PDB	External Database	Details
Met 239	-	expression tag
Gly 240	-	expression tag
Ser 241	-	expression tag
Ser 242	-	expression tag
His 243	-	expression tag
His 244	-	expression tag
His 245	-	expression tag
His 246	-	expression tag
His 247	-	expression tag
His 248	-	expression tag
Ser 249	-	expression tag
Ser 250	-	expression tag
Gly 251	-	expression tag
Leu 252	-	expression tag
Val 253	-	expression tag
Pro 254	-	expression tag
Arg 255	-	expression tag
Gly 256	-	expression tag
Ser 257	-	expression tag

B: -2 - 74 (UniProt: P0CG48)

PDB	External Database	Details
Met -16	-	expression tag
Ala -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
Ser -8	-	expression tag
Ser -7	-	expression tag
Gly -6	-	expression tag
Leu -5	-	expression tag
Val -4	-	expression tag
Pro -3	-	expression tag
Ser 0	Gly 76	engineered mutation
Asn 6	Lys 82	engineered mutation
Thr 11	Lys 87	engineered mutation
His 12	Thr 88	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	52501.4
Non-Polymers*	Number of molecules	4
	Total formula weight	228.4
All*	Total formula weight	52729.8

*Water molecules are not included.