3V2A

VEGFR-2/VEGF-A COMPLEX STRUCTURE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9999
Spacegroup name	P 65 2 2
Unit cell lengths	80.969, 80.969, 332.063
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	68.608 - 3.204
R-factor	0.2471
Rwork	0.244
R-free	0.30050
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	VEGF-E and VEGFR-2 VEGF-C complex structure
RMSD bond length	0.004
RMSD bond angle	0.834
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.7_650))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	68.608	3.400
High resolution limit [Å]	3.200	3.200
<I/σ(I)>		1.25
Completeness [%]	95.9	94.1
Redundancy	2.1	2.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	0.1 M NaCl, 1.6 M ammonium sulfate, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K