3V1G
Forestalling insulin fibrillation by insertion of a chiral clamp mechanism-based application of protein engineering to global health
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-01-14 |
| Detector | Bruker AXIOM 200 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | H 3 |
| Unit cell lengths | 79.081, 79.081, 37.402 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 39.540 - 2.200 |
| Rwork | 0.229 |
| R-free | 0.32300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1trz |
| RMSD bond angle | 0.008 |
| Data reduction software | PROTEUM PLUS |
| Data scaling software | PROTEUM PLUS |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.540 | 2.340 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.036 | 0.232 |
| Number of reflections | 4321 | |
| <I/σ(I)> | 16.9 | 3.7 |
| Completeness [%] | 98.4 | 100 |
| Redundancy | 3.2 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8 | 298 | 0.02 M Tris, 0.05 M sodium citrate, 5% acetone, 0.03% phenol, 0.01% zinc acetate, pH 8.0, VAPOR DIFFUSION, temperature 298K |
