3UWP

Crystal structure of Dot1l in complex with 5-iodotubercidin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	CCD
Collection date	2011-06-23
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.91963
Spacegroup name	P 65
Unit cell lengths	149.784, 149.784, 53.521
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	40.000 - 2.050
R-factor	0.196
Rwork	0.195
R-free	0.22310
RMSD bond length	0.013
RMSD bond angle	1.327
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.120
High resolution limit [Å]	2.050	4.420	2.050
Rmerge	0.098	0.046	0.973
Number of reflections	43269
<I/σ(I)>	8.8
Completeness [%]	99.6	99.5	96.7
Redundancy	5.9	6.1	4.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	4.6	291	3.5M sodium formate, 0.1M sodium acetate, pH 4.6, vapor diffusion, temperature 291K