3UVN
Crystal structure of WDR5 in complex with the WDR5-interacting motif of SET1A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-D |
Synchrotron site | APS |
Beamline | 21-ID-D |
Temperature [K] | 200 |
Detector technology | CCD |
Collection date | 2011-06-10 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.12718 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 64.890, 47.100, 102.520 |
Unit cell angles | 90.00, 108.21, 90.00 |
Refinement procedure
Resolution | 42.401 - 1.792 |
R-factor | 0.1953 |
Rwork | 0.194 |
R-free | 0.23030 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2h13 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.190 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASES |
Refinement software | BUSTER (2.8.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 42.401 | 2.200 |
High resolution limit [Å] | 1.792 | 1.792 |
Number of reflections | 68329 | |
<I/σ(I)> | 22 | 4.8 |
Completeness [%] | 98.1 | 91.1 |
Redundancy | 7 | 2.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 298 | 25% PEG3350, 0.1 M sodium acetate, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |