3US2
Structure of p63 DNA Binding Domain in Complex with a 19 Base Pair A/T Rich Response Element Containing Two Half Sites with a Single Base Pair Overlap
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-06-10 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.03320357 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 119.739, 180.544, 98.174 |
Unit cell angles | 90.00, 90.05, 90.00 |
Refinement procedure
Resolution | 59.869 - 4.200 |
R-factor | 0.3402 |
Rwork | 0.326 |
R-free | 0.33420 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.018 |
RMSD bond angle | 1.747 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.7_650)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 99.788 | 4.300 |
High resolution limit [Å] | 4.200 | 4.200 |
Rmerge | 0.112 | 0.453 |
Number of reflections | 14781 | |
<I/σ(I)> | 5.8 | 1.6 |
Completeness [%] | 96.8 | 94.6 |
Redundancy | 2.8 | 2.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.8 | 298 | 0.2 M ammonium formate, 0.1 M Bis-Tris, pH 6.8, 12% PEG3350, VAPOR DIFFUSION, HANGING DROP, temperature 298K |