3UQ0

Crystal structure of the post-catalytic product complex of polymerase lambda with an rAMP at the primer terminus.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	CCD
Collection date	2011-01-07
Detector	RIGAKU SATURN 92
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	55.843, 64.241, 139.290
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.916 - 2.140
R-factor	0.1968
Rwork	0.195
R-free	0.23890
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	3mgi
RMSD bond length	0.006
RMSD bond angle	0.940
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((phenix.refine: 1.7_650))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.230
High resolution limit [Å]	2.140	2.140
Number of reflections	27507
<I/σ(I)>	20.4	6.2
Completeness [%]	97.2	90.1
Redundancy	8.6	5.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	100mM Na HEPES pH 7.5, 1.0M lithium sulfate, VAPOR DIFFUSION, HANGING DROP, temperature 293K