3UQ0
Crystal structure of the post-catalytic product complex of polymerase lambda with an rAMP at the primer terminus.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-01-07 |
Detector | RIGAKU SATURN 92 |
Wavelength(s) | 1.5418 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 55.843, 64.241, 139.290 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 25.916 - 2.140 |
R-factor | 0.1968 |
Rwork | 0.195 |
R-free | 0.23890 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 3mgi |
RMSD bond length | 0.006 |
RMSD bond angle | 0.940 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX ((phenix.refine: 1.7_650)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.230 |
High resolution limit [Å] | 2.140 | 2.140 |
Number of reflections | 27507 | |
<I/σ(I)> | 20.4 | 6.2 |
Completeness [%] | 97.2 | 90.1 |
Redundancy | 8.6 | 5.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 100mM Na HEPES pH 7.5, 1.0M lithium sulfate, VAPOR DIFFUSION, HANGING DROP, temperature 293K |