3UPF
Crystal structure of murine norovirus RNA-dependent RNA polymerase bound to NF023
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-02-17 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97625 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 120.860, 195.950, 109.240 |
| Unit cell angles | 90.00, 114.38, 90.00 |
Refinement procedure
| Resolution | 45.000 - 2.600 |
| R-factor | 0.20167 |
| Rwork | 0.199 |
| R-free | 0.26062 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.267 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.000 | 2.740 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Number of reflections | 70871 | |
| Completeness [%] | 100.0 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.4 | 293 | 1.5 M ammonium sulfate, 11 % glycerol, 50 mM TRIS pH 8.4, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
