3UOA
Crystal structure of the MALT1 paracaspase (P21 form)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-06-28 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97891 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 63.475, 75.735, 87.831 |
| Unit cell angles | 90.00, 88.98, 90.00 |
Refinement procedure
| Resolution | 36.415 - 1.750 |
| R-factor | 0.1957 |
| Rwork | 0.194 |
| R-free | 0.22550 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.063 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.6.4_486) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.810 |
| High resolution limit [Å] | 1.750 | 3.770 | 1.750 |
| Rmerge | 0.062 | 0.037 | 0.483 |
| Number of reflections | 83571 | ||
| <I/σ(I)> | 8.5 | ||
| Completeness [%] | 99.4 | 98 | 99.1 |
| Redundancy | 3.8 | 3.7 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 0.1M MES (pH 6.0), 0.2M calcium acetate, 2% benzamidine, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
