3UMY
Crystal structure of mutant ribosomal protein T217A TthL1 in complex with 80nt 23S RNA from Thermus thermophilus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | BRUKER AXS MICROSTAR |
Temperature [K] | 110 |
Detector technology | IMAGE PLATE |
Collection date | 2011-04-21 |
Detector | MAR scanner 345 mm plate |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 67.110, 68.820, 88.830 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 27.200 - 1.900 |
R-factor | 0.199 |
Rwork | 0.196 |
R-free | 0.24600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3u4m |
RMSD bond length | 0.009 |
RMSD bond angle | 1.139 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.7.2_869)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 27.200 | 2.000 |
High resolution limit [Å] | 1.900 | 1.900 |
Number of reflections | 32762 | |
<I/σ(I)> | 10.99 | 2.73 |
Completeness [%] | 98.9 | 98.2 |
Redundancy | 3.54 | 3.51 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6.5 | 295 | 2.0 M Ammonium sulfate, 0.1 M Bis-Tris, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |