Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-02-12 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.54 |
| Spacegroup name | I 4 |
| Unit cell lengths | 121.531, 121.531, 67.909 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 31.579 - 2.343 |
| R-factor | 0.1831 |
| Rwork | 0.180 |
| R-free | 0.22770 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gdd |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.078 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: dev_887)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.580 | 2.360 |
| High resolution limit [Å] | 2.340 | 2.340 |
| Rmerge | 0.071 | 0.898 |
| Number of reflections | 19799 | |
| <I/σ(I)> | 27.7 | 2 |
| Completeness [%] | 94.5 | 67.4 |
| Redundancy | 4.2 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.5 | 291 | Protein: 10-20 mg/mL in 50 mM HEPES, pH 8.0, 1 mM EDTA, 100 microM GDP, 5 mM DTT, well solution: 1.8-2.2 M ammonium sulfate, 0.1M sodium acetate, pH 6.0, VAPOR DIFFUSION, temperature 291K |
