3UMI
X-ray structure of the E2 domain of the human amyloid precursor protein (APP) in complex with zinc
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.2 |
Synchrotron site | BESSY |
Beamline | 14.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-06-04 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1.28248 |
Spacegroup name | P 43 |
Unit cell lengths | 39.757, 39.757, 126.425 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 27.440 - 2.400 |
R-factor | 0.204 |
Rwork | 0.204 |
R-free | 0.24500 |
Structure solution method | SAD |
RMSD bond length | 0.005 |
RMSD bond angle | 0.948 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | SHARP |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 27.440 | 2.530 |
High resolution limit [Å] | 2.400 | 2.400 |
Number of reflections | 7453 | |
Completeness [%] | 96.8 | 89 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.4 | 293 | 0.1 mM HEPES, 1 M sodium acetate, 10 mM MgCl2, 50 mM CdSO4, pH 6.4, VAPOR DIFFUSION, SITTING DROP, temperature 293K |