3UK6
Crystal Structure of the Tip48 (Tip49b) hexamer
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-06-14 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9720 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 113.070, 186.280, 129.860 |
Unit cell angles | 90.00, 108.95, 90.00 |
Refinement procedure
Resolution | 43.190 - 2.950 |
R-factor | 0.2425 |
Rwork | 0.241 |
R-free | 0.26420 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 1.190 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | BUSTER (2.10.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 43.190 | 3.030 |
High resolution limit [Å] | 2.950 | 2.950 |
Rmerge | 0.054 | 0.690 |
Number of reflections | 103557 | |
<I/σ(I)> | 16.7 | 1.91 |
Completeness [%] | 97.0 | 99.2 |
Redundancy | 3.3 | 3.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 0.1M Tris-HCL, pH 8.0, 20% PEG 3000, 5% glucose, galactose or trehalose, VAPOR DIFFUSION, SITTING DROP, temperature 293K |